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Methylfolate (L-5-MTHF)

Methylation · mood · MTHFR support

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Written to our editorial standards · reviewed against published research· Updated 2 Jun 2026
Last reviewed: May 30, 2026
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Methylfolate: Methylation · mood · MTHFR support. The bioactive form of folate, bypasses the MTHFR enzyme, supporting methylation, mood, and homocysteine balance. Standard dose 1 mg, taken in the morning, about $16/month. Evidence rating: strong evidence.

What is Methylfolate?

L-5-MTHF (L-methylfolate) is the biologically active, ready-to-use form of folate (vitamin B9). Unlike synthetic folic acid, which requires conversion through the MTHFR enzyme, methylfolate is absorbed and used directly, making it the preferred form for people with common MTHFR gene variants (estimated to affect 30-50% of the population). Methylfolate is essential for methylation, the biochemical 'on/off' switch that regulates DNA expression, neurotransmitter synthesis, and homocysteine recycling. Adequate methylfolate supports mood, cognition, cardiovascular health, and is critical during pregnancy. Standard doses are 400 mcg-1 mg per day.

WHY IT MATTERS

The bioactive form of folate, bypasses the MTHFR enzyme, supporting methylation, mood, and homocysteine balance.

Natural food sources

Where possible, get Methylfolate from whole foods first. Common dietary sources include:

Leafy greens (spinach)LentilsAsparagusAvocadoLiver

Where to buy

Thorne 5-MTHF 1mg
RECOMMENDED
Thorne, Methylfolate
1 mg · Morning · ~$16/month

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Common questions

What is Methylfolate?
L-5-MTHF (L-methylfolate) is the biologically active, ready-to-use form of folate (vitamin B9). Unlike synthetic folic acid, which requires conversion through the MTHFR enzyme, methylfolate is absorbed and used directly, making it the preferred form for people with common MTHFR gene variants (estimated to affect 30-50% of the population). Methylfolate is essential for methylation, the biochemical 'on/off' switch that regulates DNA expression, neurotransmitter synthesis, and homocysteine recycling. Adequate methylfolate supports mood, cognition, cardiovascular health, and is critical during pregnancy. Standard doses are 400 mcg-1 mg per day.
What is Methylfolate used for?
Methylation · mood · MTHFR support. The bioactive form of folate, bypasses the MTHFR enzyme, supporting methylation, mood, and homocysteine balance.
What is the standard dose of Methylfolate?
1 mg, typically taken in the morning. Approximate cost is $16 per month.
How strong is the evidence for Methylfolate?
Strong evidence for its primary uses. See the full study list on the research page.

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